EUBCE 2024 - Giacomo FABBRIZI - Optimizing Biorefinery Efficiency: High-Solid, Low-Dosage Enzymatic Hydrolysis of Ethanol-Based Acid-Catalyzed Organosolv of Cynara Cardunculus

Optimizing Biorefinery Efficiency: High-Solid, Low-Dosage Enzymatic Hydrolysis of Ethanol-Based Acid-Catalyzed Organosolv of Cynara Cardunculus

Short Introductive summary

Cynara cardunculus is a perennial non-food oilseed crop native of the Mediterranean region able to grow in marginal lands with a relatively low crop input and a large biomass productivity. Its stalks (CcS) are a residual lignocellulosic biomass commonly used as a fuel in combustion process. In this work, pretreatment of CcS with microwaved-assisted, acid-catalyzed, ethanol-based organosolv was used to produce a cellulose pulp that underwent a high-solid low-dosage enzymatic hydrolysis to valorize the saccharide content. Two different Experimental Designs based on Response Surface Methodology were applied to respectively check acid catalyst concentration, ethanol/water mixture concentration, and temperature in the organosolv process, and enzyme concentration, solid loading, and incubation time in the enzymatic hydrolysis, defining an optimal point for both processes.

G. Fabbrizi, Università degli studi di Perugia, ITALY
G. Russo, Università degli studi di Perugia, ITALY
F. Spaccini, Università degli studi di Perugia, ITALY
T. Giannoni, Università degli studi di Perugia, ITALY
M. Gelosia, Università degli studi di Perugia, ITALY
A. Nicolini, Università degli studi di Perugia, ITALY