EUBCE 2024 - Eun Jin CHO - Extraction, Characterization and Anti-obesity Effects of Manno-oligodaccharide (MOS) Derived from Spent coffee Grounds

Extraction, Characterization and Anti-obesity Effects of Manno-oligodaccharide (MOS) Derived from Spent coffee Grounds

Short Introductive summary

Spent coffee grounds (SCG) contain a high amount of mannan, making them crucial for the production of nutraceutical manno-oligosaccharides (MOS). There has been a growing interest in producing MOS for health promotion in both humans and animals due to their reported various bioactive properties, including prebiotic and antioxidant activities. In this study, mannan was isolated from SCG using two different extraction methods. Structural analyses, employing FT-IR and solid 13C NMR, were conducted to assess the efficacy of the extraction methods. The isolated mannan was efficiently hydrolyzed by a recombinant ß-mannanase. Conditions for MOS production were optimized by varying the reaction temperature (40°C–80°C) and time (10–120 min). The optimal conditions were found to be 80°C and 90 min, respectively, resulting in the highest concentration of total MOS. The hydrolysis of mannan by a recombinant ß-mannanase produced mannobiose (M2) and mannotriose (M3) as the main MOS products. Subsequently, we investigated the anti-obesity effects of MOS in a high-fat diet (HFD) mouse model. After 6 weeks of HFD+MOS, we evaluated HFD-induced total fat, subcutaneous fat, and visceral fat. The exper

E.J. Cho, Chonnam National University, Gwangju, REPUBLIC OF KOREA
H.-J. Bae, Chonnam National University, Gwangju, REPUBLIC OF KOREA
S.H. Jang, Chonnam National University, Gwangju, REPUBLIC OF KOREA