EUBCE 2024 - Fernanda THIMOTEO AZEVEDO JORGE - Biotechnological Production of 2-Phenylethanol from Agro-Industrial Wastes for Foods and Flavouring Applications

Biotechnological Production of 2-Phenylethanol from Agro-Industrial Wastes for Foods and Flavouring Applications

Short Introductive summary

This study evaluates the use of apple pomace and whey as low-cost, bio-based substrates for the growth of the yeast strain Kluyveromyces marxianus CBS600, used for sustainable production of a high value aroma compound, 2-phenylethanol (2-PE). Among the waste streams used as carbon and energy source for yeast growth, apple pomace resulted in the highest 2-PE productivity in shake flasks, reaching a concentration of 1.07 g/L in 24 h, while its upscaling to a 1 L bioreactor led to 1.60 g/L in 68 h. In addition, fermentation of acid whey supplemented with yeast extract and minerals for growth purposes significantly improved the bioconversion, leading to 2.64 g/L of 2-PE in 72 h. Furthermore, a techno-economic assessment showed that 2-PE production from apple pomace displays a high profitability, with a unit production cost of US$245/kg and an internal rate of return of 13.8%. Hence, this study shows that 2-PE production by fermentation is a sustainable and economically viable process that can contribute to a circular bioeconomy by valorizing agro-industrial residues.

F. Thimoteo Azevedo Jorge, AgroParisTech, Palaiseau, FRANCE
N. Orynbek, AgroParisTech, Palaiseau, FRANCE
A. Abduraimova, AgroParisTech, Palaiseau, FRANCE
A. Valakhanovich, AgroParisTech, Palaiseau, FRANCE
P. Jacques, University of Liège, Gembloux, BELGIUM
L. Besaury, Université de Reims Champagne Ardenne, Reims, FRANCE
M. Moussa, AgroParisTech, Palaiseau, FRANCE
C. Saulou-Bérion, AgroParisTech, Palaiseau, FRANCE
A.K. Sánchez-Castañeda, AgroParisTech, Palaiseau, FRANCE