Date: Thursday, 12 May 2022
Time: 12:50 - 13:40 CEST
Session code 6CV.7
Biorefinery Platforms for Bio-based Chemicals and Polymers
Valorisation of Brewer’s Spent Grain for Poly(3-hydroxybutyrate) Production Via Fermentation
Short Introductive summary
The BSG, the main by-product derived from the brewing industry, can be considered as a promising feedstock for the biotechnological production of bio-based P3HB. It comprises a material with high carbohydrates content, resulting in hydrolysates rich in fermentable sugars. An efficient enzymatic hydrolysis strategy has been developed by employing a mixture of different commercial enzymes. The produced hydrolysate consists of glucose (56 %), xylose (38 %) and arabinose (6 %). Efficient P3HB production has been achieved when neutral and alkaline pre-treatment of BSG, prior to enzymatic hydrolysis, was performed. DSP process for the extraction of intracellular P3HB has been developed with the utilization of green solvents, leading to high P3HB purity and recovery yields if compared to the conventional methods. The most promising results were obtained when enzymatic cell lysis was employed prior to 1,3-dioxolane treatment. As future work during the BioSupPack project, air cold plasma BSG-pretreatment will be developed, targeting the removal of lignin fraction in order for the subsequent enzymatic hydrolysis of cellulose and hemicellulose to be more efficient.
Presenter
Chrysanthi ARGEITI
Agricultural University of Athens, Food Science and Human Nutrition, Agricultural University of Athens
Biographies and Short introductive summaries are supplied directly by presenters and are published here unedited
Co-authors:
S. Obruca, Faculty of Chemistry Brno University of Technology, Brno, CZECH REPUBLIC
R. González, AIMPLAS, Valencia, SPAIN
C. Pateraki, Agricultural University of Athens, GREECE
A. Cordes, Asa Spezialenzyme, Wolfenbüttel, GERMANY
M. Scauri, ENCO, Napoli, ITALY
M. Kryl, Nafigate Corporation, Praha, CZECH REPUBLIC
D. Ladakis, Agricultural University of Athens, GREECE
Marco De la Feld, ENCO, Napoli, ITALY
P. Ferrero, AIMPLAS, Valencia, SPAIN
R. Giner Borrull, AIMPLAS, Valencia, SPAIN
C. Argeiti, Agricultural University of Athens, GREECE
A. Pizza, ENCO, Napoli, ITALY
A. Koutinas, Agricultural University of Athens, GREECE
Session reference: 6CV.7.12