EUBCE 2024 - Hyeunjong BAE - Production of Chito-Oligosacchariedes from Various Biomass Using Immobilized Chitosanase Enzyme

Production of Chito-Oligosacchariedes from Various Biomass Using Immobilized Chitosanase Enzyme

Short Introductive summary

Chito-oligosaccharides, derived from chitosan degradation, exhibit a wide range of properties, including low toxicity, high biodegradability, and diverse biological functions. In this study, chitin and chitosan were extracted from crab and shrimp waste through chemical extraction. Subsequently, chitosanase enzymes from Bacillus subtilis and Bacillus theringeinsis were immobilized on chitin resin using the chitin-binding domain (CBD). The immobilization process involved degrading chitosan using immobilized enzymes. This innovative approach allows for the production of chito-oligosaccharides with varying sizes. The enzyme activity temperature and substrate flow rate were controlled to achieve this, with optimal conditions identified as an enzyme activity temperature of 25°C and a substrate flow rate of approximately 1.0 mL/min. Under these conditions, chitosanase produced chito-oligosaccharides ranging from a degree of polymerization (DP) of 2-7. Chito-oligosaccharides present an eco-friendly solution for utilizing crab and shrimp waste, finding applications in food production, agriculture, and water purification processes. The use of chitin resin for enzyme immobilization enhances t

E.S. Kim, Chonnam National University, Gwangju, REPUBLIC OF KOREA
H.-J. Bae, Chonnam National University, Gwangju, REPUBLIC OF KOREA