EUBCE 2024 - Apostolos PETROPOULOS - Succinic Acid Production Via Fermentation with an Engineered Escherichia Coli Strain using Biowaste Fractions and Softwood Sawdust Hydrolysates

Succinic Acid Production Via Fermentation with an Engineered Escherichia Coli Strain using Biowaste Fractions and Softwood Sawdust Hydrolysates

Short Introductive summary

This study evaluates the production of succinic acid (SA) using OFMSW and sawdust hydrolysates with employing the bacterial strain Escherichia coli AFP184 in a two-stage cultivation process. The OFMSW and sawdust streams were subjected to enzymatic hydrolysis using commercial enzymes to produce crude hydrolysates. These raw materials possess a high carbohydrate content, leading to hydrolysates rich in fermentable sugars and nutrients that promote efficient microbial growth. The effective use of OFMSW and sawdust hydrolysates for SA production by the bacterial strain E. coli AFP184, along with the viability of SA production processes, holds the potential to greatly enhance the profitability of bio-based SA production across diverse feedstocks.

A. Petropoulos, Agricultural University of Athens, GREECE
C. Margioulas, Agricultural University of Athens, GREECE
D. Ladakis, Agricultural University of Athens, GREECE
E. Sstylianou, Agricultural University of Athens, GREECE
A. Koutinas, Agricultural University of Athens, GREECE