Room: Yangtze 1
Date: Tuesday, 19 May 2026
Time: 15:00 - 16:00 CEST
Session code 6AO.3
Bioprocess innovations for biomass valorization
Evaluation of 2,3-Butanediol Production using Brewer’s Spent Grains and Acid Whey in a Co-culture System
Short Introductive summary
This project evaluates a synergistic co-culture system composed of Pseudomonas putida and Paenibacillus polymyxa for the sustainable production of 2,3-butanediol (2,3-BDO) from brewer’s spent grain (BSG) and acid whey. In this system, P. putida functions as a biological detoxifier, removing inhibitory compounds generated during the acid hydrolysis of BSG, while P. polymyxa metabolises fermentable sugars, such as glucose, xylose, galactose, and arabinose, into 2,3-butanediol (2,3-BDO). The study integrates the liquid and solid fractions of pretreated BSG with varying concentrations of acid whey in a simultaneous saccharification and fermentation process, comparing mono- and co-culture conditions. The results demonstrate a significant improvement in inhibitor removal and higher 2,3-BDO titers under co-culture conditions, highlighting its potential as a biotechnological strategy for the valorization of lignocellulosic biomass and the sustainable production of bio-based compounds.
Presenter
Maria Fernanda RAMOS REYES
DTU, DENMARK
Presenter's biography
I began my academic training in Pharmaceutical Chemistry at the technical level, later shifting my focus toward the engineering aspects of biotechnology during my bachelor’s and master’s studies. Over time, I developed a strong interest in sustainability and circular economy approaches.
Biographies and Short introductive summaries are supplied directly by presenters and are published here unedited
Co-authors:
L. Gram, DTU, Kogens Lyngby, DENMARK
C. Senés, Tecnológico de Monterrey, Zapopan, MEXICO
S.I. Mussatto, DTU, Kogens Lyngby, DENMARK
Session reference: 6AO.3.2