Room: Yangtze 1
Date: Tuesday, 19 May 2026
Time: 16:15 - 17:15 CEST
Session code 6AO.6
Processes and technologies for biochemical and biomaterials production
Integrated Enzymatic Valorization of Brewer’s Spent Grain for Protein and Phenolic Recovery
Short Introductive summary
Brewer’s spent grain (BSG), the main by-product of the brewing industry, is a valuable source of proteins and phenolic compounds. This study investigated enzymatic hydrolysis using Alcalase, Flavourzyme, and their combinations to enhance protein recovery while enabling the separation of phenolic constituents. The process was optimized for enzyme concentration, pH, and reaction time at a 300 mL laboratory scale. Under optimal conditions, protein extraction yields reached 95–98% (22.5 ± 0.1 g/100 g dry BSG), and phenolic content of 1.3 g GAE/100 g dry BSG was obtained after C18 solid-phase extraction. The integrated method demonstrates an efficient and sustainable strategy for the dual valorization of BSG, contributing to circular bioeconomy goals and offering potential for future industrial application in food and nutraceutical formulations.
Presenter
Sarah DE SOUZA QUEIROZ
Technical University of Denmark, DENMARK
Presenter's biography
Researcher at Technical University of Denmark (DTU), working on lignocellulosic biomass valorization through biological and biochemical conversion processes. Her research focuses on biomass pretreatment, microbial fermentation, metabolic engineering, and bioprocess optimization.
Biographies and Short introductive summaries are supplied directly by presenters and are published here unedited
Co-authors:
O. Gouseti, University of Copenhagen, DENMARK
S. Bakalis, University of Copenhagen, DENMARK
G. Dragone, University of Copenhagen, DENMARK
S. Mussatto, Technical University of Denmark, Kongens Lyngby, DENMARK
Session reference: 6AO.6.2